Thank you for this, I've wanted to do genetic testing for years but don't want it to be stored on anyone's server. Can you explain how it's not stored on any server? Like, you're downloading the .BAM file from Nebula; doesn't that mean they hold onto the data?
Genetic Life Hacks provides the analysis on a number of health and disease related topics. Their analysis is simply allele matching for any given topic (string comparisons). It's all done within the browser.
Anyone who does the actual sequencing has the data. I think they mean the final service that does the analysis runs locally in the browser. You still need to trust whoever does the sequencing.
Your identity can be reconstructed from relative's samples. If you submit your sequence its out of your hands forever. The only way to do this privately is to do the sequencing in your own lab.
I sequence my own genome in my own private lab. At least weekly. The rush when the sequencing begins. I almost can't help but sequence all over the place.
23andme has a "report" about this called Caffeine Consumption. The markers they use are rs4410790 and rs2472297, whereas geneticlifehacks.com talks about rs762551. I'm not sure what to make of that, since that second 23andme one is "near" cyp1a2.
When I think about sequencing, I think about verifiability. With anonymity blinkers on, you could give me several gigabytes of random data and I'd have limited recourse.
Will Nebula give me an intelligible whole-genome data file if I happen to be a Golden Retriever?
You could check it against things that you know about yourself.
I know from my clinical history that I have mutations in the UGT1A1 gene that cause me to metabolize bilirubin differently. That is a relatively common mutation that occurs in roughly 2-3 percent of people. Unless I am lucky enough to have a random result that coincides with this, I could be fairly certain that
this data is somewhat reliable. The problem thought, I don't know the degree of reliability.
Wait for a sale at Nebula.org. Get your whole genome sequenced. ~$200. Having your whole genome sequenced could have added benefits down the road.
Download the .BAM file. It will be several gigabytes.
Use WSG Extract to generate the 23andMe raw data files.
https://www.geneticlifehacks.com/convert-whole-genome-file/
Connect that data file to Genetic Life Hacks. Take a look at the article on CYP1A2. This will show you whether you're a slow metabolizer or not.
https://www.geneticlifehacks.com/liver-detox-genes-cyp1a2/
The linked data file at GLF never uploads to the server. All analysis is local, client side.